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A convenient method for the ATPase assay

✍ Scribed by Denis LeBel; Guy G. Poirier; Adrien R. Beaudoin

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
232 KB
Volume
85
Category
Article
ISSN
0003-2697

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✦ Synopsis

A new method for the determination of inorganic phosphorus released in ATPase assay has been evaluated. The method is based on the reduction of a phosphomolybdate complex by Elon in a copper acetate buffer. In contrast to current methods, there is no interference by ATP with color development. There is also less or no interference by other compounds usually present in ATPase assay media. The method is simple, sensitive, and reproducible.

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