𝔖 Bobbio Scriptorium
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A convenient method for isolating the disaccharide and the tetrasaccharide in muramidase digests of Micrococcus lysodeikticus cell walls

✍ Scribed by John C. Speck Jr.; Donald J. Rynbrandt

Book ID
102625997
Publisher
Elsevier Science
Year
1967
Tongue
English
Weight
487 KB
Volume
19
Category
Article
ISSN
0003-2697

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✦ Synopsis

We describe a simple method for preparative-scale isolation of the disaccharide and the tetrasaccharide3 in muramidase digests of Mwro-G~~GUS lysodeiktims cell walls. This procedure, developed to meet a need in this laboratory for gram amounts of the tetrasaccharide, consists of only two steps: (I) removing basic and, possibly, certain neutral substances from dialyzates of 12 to 24 hr muramidase digests of the cell walls on the hydrogen ion form of a sulfonated polystyrene resin (Amberlite CG-120) and (5') fractionating the substances that are not absorbed on this resin by gel filtration through Sephadex G-15, monitoring the effluent in this step by measuring its absorbance at 230 my in a spectrometer equipped with flow cell and recorder.

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