A calibration procedure for a Clark-type oxygen electrode over a wide range of temperatures is described. The autoxidation of duroquinol (2,3,5,6-tetramethyl-1,4-benzenediol) was used to verify the electrode's ability to accurately sense the total amount of dissolved O2 in an aqueous buffer. Electro
A convenient calibration of the Clark oxygen electrode
β Scribed by Hara P. Misra; Irwin Fridovich
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 145 KB
- Volume
- 70
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
A procedure for calibrating a Clark-type oxygen electrode is described. This method is based on the oxidation of 4-tert-butylcatechol (TBC) by O2 catalyzed by tyrosinase, to yield 4-tert-butyl-o-benzoquinone (TBCQ). This reaction consumes known amounts of oxygen in accordance with the stoichiometry:
A Clark-type oxygen electrode with three electrodes has been fabricated using semiconductor techniques and its characteristics compared with those of double oxygen electrodes with a similar structure. The 90% response time is 40 s, the residual current at zero oxygen concentration is less than 7%, a