A continuous ultrasound-assisted packed-bed bioreactor for the lipase-catalyzed synthesis of caffeic acid phenethyl ester
✍ Scribed by Hsiao-Ching Chen; Chia-Hung Kuo; Yawo-Kuo Twu; Jiann-Hwa Chen; Chieh-Ming J. Chang; Yung-Chuan Liu; Chwen-Jen Shieh
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2011
- Tongue
- English
- Weight
- 141 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0268-2575
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✦ Synopsis
Abstract
BACKGROUND: The focus of this paper is the ultrasound‐assisted synthesis of caffeic acid phenethyl ester (CAPE) from caffeic acid and phenyl ethanol in a continuous packed‐bed bioreactor. Immobilized Novozym^®^ 435 (from Candida antarctica) is used as the catalyst. A three‐level–three‐factor Box–Behnken design and a response surface methodology (RSM) are employed to evaluate the effects of temperature, flow rate, and ultrasonic power on the percentage molar conversion of CAPE.
RESULTS: Based on ridge max analysis, it is concluded that the optimum condition for synthesis is reaction temperature 72.66 °C, flow rate 0.046 mL min^−1^, and ultrasonic power 1.64 W cm^−2^. The expected molar conversion value is 97.84%. An experiment performed under these optimal conditions resulted in a molar conversion of 92.11 ± 0.75%. The enzyme in the bioreactor was found to be stable for at least 6 days.
CONCLUSIONS: The lipase‐catalyzed synthesis of CAPE by an ultrasound‐assisted packed‐bed bioreactor uses mild reaction conditions. Enzymatic synthesis of CAPE is suitable for use in the nutraceutical and food production industries. Copyright © 2011 Society of Chemical Industry