A Continuous Spectrophotometric Assay for 5-Aminolevulinate Synthase That Utilizes Substrate Cycling

A Continuous, Anaerobic Spectrophotometr

A Continuous, Anaerobic Spectrophotometric Assay for Chorismate Synthase Activity That Utilizes Photoreduced Flavin Mononucleotide

✍ M.K. Ramjee; J.R. Coggins; R.N.F. Thorneley 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 364 KB

A sensitive, continuous, spectrophotometric assay for chorismate synthase has been developed utilizing photoreduced flavin mononucleotide \(\left(\mathbf{F M N H}_{2}\right.\) ) as a cofactor under anaerobic conditions. The assay monitors directly the formation of chorismate from 5-enolpyruvylshikim