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A continuous fluorescence assay for the characterization of Nudix hydrolases

✍ Scribed by Xu, Anting; Desai, Anna M.; Brenner, Steven E.; Kirsch, Jack F.

Book ID
122281191
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
622 KB
Volume
437
Category
Article
ISSN
0003-2697

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Peptidyl-tRNA hydrolase (Pth) activity ensures the rapid recycling of peptidyl-tRNAs that result from premature termination of translation. Historically, the hydrolyzing activity of Pth has been assayed with radiolabeled N-blocked aminoacyl-tRNAs in assay systems that require the separation of radio