A computer-assisted rapid-scanning spectrophotometer with applications to tissues in vitro and in vivo
✍ Scribed by S.M. Pikarsky; J.C. LaManna; T.J. Sick; M. Rosenthal
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 839 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0010-4809
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✦ Synopsis
A flexible approach is described to acquire, store, retrieve, and manipulate the large volume of data provided by a rapid-scanning spectrophotometer designed for biological preparations. Data can be displayed in spectral and kinetic formats. This spectrophotometer provides a means to simultaneously measure the concentrations of components of light scattering preparations and to record chemical reactions as they occur. Although application of this instrument for the study of reduction/oxidation shifts of mitochondrial respiratory chain components is emphasized, the design is suitable for many spectrophotometric applications.