## Abstract We describe the application of a general method of comparing and sorting the patterns of protein expression obtained from two‐dimensional (2‐D) electrophoresis of a mixture of proteins. The method involved the use of two multidimensional classification programs and was applied, first, t
✦ LIBER ✦
A computer analysis of electrophoresis and ultracentrifugation patterns
✍ Scribed by E. Tränkle
- Publisher
- Elsevier Science
- Year
- 1975
- Weight
- 377 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0010-468X
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✦ Synopsis
A program DIANA is designed to fit the scanning curves of electrophoresis and ultracentrifugation patterns with 10-15 overlapping peaks by a sum of Gaussian-like distribitions. The parameters of the distributions are adjusted by minimizing x, using a problem oriented minimizing procedure. The number of molecular components as well as starting values of the positions and the widths of the peaks must be choosen as input for each type of pattern. Programs are written in FORTRAN IV.
Electrophoresis
Ultracentrifugation patterns Least-square fit
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