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A comprehensive study of the loss of enzyme activity in a continuous membrane reactor – application to starch hydrolysis

✍ Scribed by Delphine Paolucci-Jeanjean; Marie-Pierre Belleville; Gilbert M Rios

Publisher: Wiley (John Wiley & Sons)
Year: 2001
Tongue: English
Weight: 157 KB
Volume: 76
Category: Article
ISSN: 0268-2575
DOI: 10.1002/jctb.380

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✦ Synopsis

Abstract

Loss of enzyme activity is a problem associated with enzymatic reactions in continuous recycled membrane reactors (CRMR). It may result from catalyst leakage and also enzyme denaturation due to the effects of pH, temperature, shear effects or adsorption/deposit on membrane. In this study, the relative importance of these various factors has been assessed in order to reduce their adverse effects on starch hydrolysis in a CRMR. The effects of temperature and denaturation by adsorption/deposit on membrane were the most limiting phenomena. Reducing the temperature to overcome thermal denaturation was not a practical solution since this increases viscosity and thereby decreases permeate flux and reactor performance. Insofar that adsorption/deposit of enzymes on the membrane is directly linked to membrane fouling, back‐flushing or regularly purging retentate should reduce this phenomenon by lowering accumulation of high molecular weight products.

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