A composite nerve graft system: Extracted rat peripheral nerve injected with cultured Schwann cells
✍ Scribed by Charles E. Dumont; Laurel M. Bolin; Vincent R. Hentz
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 238 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0148-639X
No coin nor oath required. For personal study only.
✦ Synopsis
have developed a method of in vitro peripheral nerve extraction in which lysophosphatidylcholine is used to remove the cells. The remaining extracellular matrix of the nerve is termed the extracted nerve graft (eNG). Cultured neonatal Schwann cells have been microinjected into these grafts, to form recellularized nerve grafts (rNG). This should improve the ability of the graft to support nerve regeneration. This study uses conventional histology and immunohistochemistry to examine the structure of the eNG and rNG, and the early host reaction to such graft implantations in rat. Both eNG and rNG have been prepared from isograft to focus this preliminary report on the biocompatibility of such a peripheral nerve implants.
Methods
Fresh sciatic nerves removed from adult F-344 rats were washed in phosphate-buffered saline and then transferred to 0.7 or 0.8% lysophosphatidylcholine for 4 days.
Primary Schwann cell cultures were established from 3-day-old F-344 rats as described by Brockes