A complex ensemble ofcis-regulatory elements controls the expression of aVicia fabanon-storage seed protein gene

✍ Scribed by Ulrike Fiedler; Roman Filistein; Ulrich Wobus; Helmut Bäumlein

• Book ID: 104622820
• Publisher: Springer
• Year: 1993
• Tongue: English
• Weight: 826 KB
• Volume: 22
• Category: Article
• ISSN: 0167-4412
• DOI: 10.1007/bf00047407

✦ Synopsis

We have identified cis-regulatory elements within the 5' -upstream region of a Viciafaba non-storage seed protein gene, called usp, by studying the expression of usp-promoter deletion fragments fused to reporter genes in transgenic tobacco seeds. 0.4 kb of usp upstream sequence contain at least six, but probably more, distinct cis-regulatory elements which are responsible for seemingly all quantitative, spatial and temporal aspects of expression. Expression-increasing and -decreasing elements are interspersed and include an AT-rich sequence, a G-box element and a CATGCATG motif. The latter acts as a negative element in contrast to what has been found for the same motif in legumin-and vicilin-type seed storage protein gene promoters. Seed specificity of expression is mainly determined by the -6 8 / + 51 region which confers, however, only very low levels of expression. The data support the combinatorial model of promoter function.