A complete BAC-based physical map of the Arabidopsis thaliana genome
β Scribed by Altmann, Thomas; Mozo, Teresa; Dewar, Ken; Dunn, Pat; Ecker, Joseph R.; Fischer, Sabine; Kloska, Sebastian; Lehrach, Hans; Marra, Marco; Martienssen, Robert; Meier-Ewert, Sebastian
- Book ID
- 109465661
- Publisher
- Nature Publishing Group
- Year
- 1999
- Tongue
- English
- Weight
- 319 KB
- Volume
- 22
- Category
- Article
- ISSN
- 1061-4036
- DOI
- 10.1038/10334
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## Summary Two regions of __Arabidopsis__ chromosome 4, totalling 4.7 Mb, were assayed for representation in the TAMU and IGF BAC libraries. A directed approach to BAC identification was developed. Gelβpurified DNA samples of YACs selected from the YACβbased physical map of chromosome 4 were used t
A new __Arabidopsis thaliana__ (ecotype Columbia) genomic library has been constructed in Yeast Artificial Chromosomes: the CIC library (for CEPH, INRA and CNRS). Optimization of plant culture conditions and protoplast preparation allowed the recovery of large amounts of viable protoplasts. Mechanic