A comparison of virus isolation, indirect immunofluorescence and nested multiplex polymerase chain reaction for the diagnosis of primary and recurrent herpes simplex type 1 and type 2 infections
✍ Scribed by P.V Coyle; A Desai; D Wyatt; C McCaughey; H.J O’Neill
- Book ID
- 114140836
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 242 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0166-0934
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## Abstract One hundred forty‐nine specimens were tested in a LightCycler nested multiplex polymerase chain reaction (LCnmPCR) for Herpes simplex virus (HSV)1, HSV2, and VZV. Eighty‐one were from genitourinary medicine (GUM) patients and the other 68 specimens were from other patients with skin les
The severity and recurrences of Herpes Simplex Virus (HSV) infection depend on the type of the infectious agent (HSV-1 or HSV-2), which induces the necessity of a nonambiguous detecting typing. The commonly used capture ELISA technique has to be often supported by DNA analysis to confirm the detecti