A comparison of Tris-glycine and Tris-tricine buffers for the electrophoretic separation of major serum proteins
✍ Scribed by Syed R. Haider; Barry L. Sharp; Helen J. Reid
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 111 KB
- Volume
- 34
- Category
- Article
- ISSN
- 1615-9306
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
This paper compares different buffer systems for the electrophoretic separation of the five most abundant serum proteins on native‐PAGE gel and cellulose membranes. A modified Tris‐tricine system was shown to be superior for the separation of these serum proteins in a 7% m/v native‐PAGE gel as compared with the traditionally used Tris‐glycine and Tris‐tricine methods. This modified Tris‐tricine buffer system was also employed for the separation of serum proteins using a cellulose acetate membrane and very effective separation was observed as compared with the traditionally used Tris‐barbital and Tris‐glycine buffer systems.