✦ LIBER ✦
A Comparison of Triazole-forming Bioconjugation Techniques for Constructing Comb-Shaped Peptide–Polymer Bioconjugates
✍ Scribed by Luiz A. Canalle; Matthijs van der Knaap; Mark Overhand; Jan C. M. van Hest
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 266 KB
- Volume
- 32
- Category
- Article
- ISSN
- 1022-1336
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✦ Synopsis
Abstract
The grafting‐to of a peptide to a side chain functional polymer was investigated using “click” chemistry. Two click reactions were compared: the copper‐free strain‐promoted azide‐alkyne 1,3‐cycloaddition (SPAAC) and the traditional copper‐catalyzed azide‐alkyne 1,3‐cycloaddition (CuAAC). For the resulting comb‐shaped products, it was found that the steric bulk of the conjugation moiety used in SPAAC limits the degree of grafting for these highly dense systems, whereas CuAAC gives (near) quantitative functionalization.
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