A comparison of thein vitro cytotoxicity of four restorative materials assessed by changes in enzyme levels in two cell types
✍ Scribed by Meryon, S. D. ;Riches, D. W. H.
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- English
- Weight
- 620 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0021-9304
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✦ Synopsis
Abstract
A system is described for assessing the toxicity of freshly mixed restorative materials in vitro by measuring changes in the levels of β‐galactosidase and lactic dehydrogenase in both cultured cells and supernatants. The toxic effects of a zinc phosphate and silicate cement, a composite, and zinc oxide/eugenol were studied on two cell types, macrophages and fibroblasts, after 24 h exposure. Zinc oxide/eugenol, Silicap, and zinc phosphate were toxic to macrophages, in that order; Concise appeared to be nontoxic. Only zinc oxide/eugenol exerted significant effects on fibroblasts. Interposing dentine powder between the test cells and the material ameliorated the effects of all materials, possibly by the absorption of toxic components.