โ Scribed by Z. Bercovich; T. Dekker; A. Eger; J. Haagsma
No coin nor oath required. For personal study only.
A comparison of the potency of several Brucella allergens used to detect brucellosis in cattle. Veterinary Research Communications, 20 (2), 141-151
The potency of Bruce/la allergens prepared from a smooth Brucella abortus strain S-99, mucoid strain Leewarden, rough strain 45120, and rough Brucella melitensis strain B-l 15 was assessed. The potency of these allergens was compared with that of a standard allergen prepared from smooth Brucelfa abortus S-99 that efficiently detected bovine brucellosis in other studies. Eight cattle experimentally inoculated with BruceZla abortus 544 were tested with the allergens 4 and 10 weeks after infection, and again 8 months after infection. All the allergens effectively detected infection but there was a clear distinction in the mean skin reactions 48 and 72 h after injection of the allergens. The skin reactions provoked by the allergens prepared from smooth or mucoid strains of Brucella were most pronounced 48 h after injection. Skin reactions provoked by allergens prepared from rough strains of BruceZZa were strongest 72 h after injection. Allergens prepared from smooth or mucoid Brucelfa strains were more potent in detecting brucellosis than those prepared from rough strains of Brucella.
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