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A comparison of the photochemical activity of two forms of Photosystem II reaction centre isolated from sugar beet

โœ Scribed by Guillermo Montoya; Javier de las Rivas; Paula J. Booth; Linda B. Giorgi; David R. Klug; George Porter; James Barber; Rafael Picorel

Book ID
115720285
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
643 KB
Volume
1185
Category
Article
ISSN
0005-2728

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A rapid procedure has been developed for the isolation of the photosystem two reaction centre complex (PS II RC) from a double mutant of Chlamydomonas reinhardtii, F54-14, which lacks the Photosystem one complex and the chloroplast ATPase. Thylakoid membranes are solubilised with 1.5% (w/v) Triton X