A comparison of the mechanism for the reductive half-reaction between pea seedling and other copper amine oxidases (CAOs)
✍ Scribed by Rajeev Prabhakar; Per E. M. Siegbahn
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 191 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0192-8651
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✦ Synopsis
Abstract
In a previous DFT study a mechanism for the reductive half‐reaction of pea seedling amine oxidase (PSAO) was suggested. In many of the suggested steps a lysine at the active site plays an important role. However, this lysine is not found in other amine oxidases. The primary aim of the present DFT study is therefore to investigate alternative mechanisms for those amine oxidases (CAO) where the lysine residue is not present. One of the most important roles suggested for the lysine in PSAO was to protonate the O2‐site of TPQ before the critical CH bond cleavage of the substrate. In the absence of lysine the O2‐site of TPQ is now suggested to be protonated by a water ligand on the copper metal complex, in line with experimental suggestions. In other steps the role of lysine is taken over by an asparagine. All results are compared with experimental observations and good agreement is generally found. © 2003 Wiley Periodicals, Inc. J Comput Chem 24: 1599–1609, 2003