Monolayers of rat hepatocytes metabolize 0.25 mM 2acetylaminofluorene (AA F) to various ether-extractable, watersoluble as well as covalently bound products. The major ether-extractable metabolite formed is 2-aminofluorene (AF), followed by 7-OH-AAF and 9-OH-AAF. Pretreatment of rats with the induce
A comparison of the inhibition of deacetylase in primary cultures of rat and human hepatocytes effecting metabolism and DNA-binding of 2-acetylaminofluorene
β Scribed by David K. Monteith; Stephen C. Strom
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 880 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0742-2091
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The metabolism and mutagenic activation of 2-acetylaminofluorene by human and rat hepatocytes and kidney cells were measured. High performance liquid chromatography was used to separate the 2-aeetylaminofluorene metabolites, and a cell-mediated Salmonella typhimurium mutagenesis assay was used to de
The effect of recombinant human hepatocyte growth factor (h-rHGF), a potent mitogen for hepatocytes, was investigated in primary cultures of human hepatocytes. Here, we describe a series of experiments to investigate the kinetics of its mitogenic action, as well as its metabolic effects on cultured
Studies were conducted to explore hormonal and nutritional factors that might be involved in the regulation of retinol-binding protein (RBP) synthesis and secretion by the liver. The studies employed primary cultures of hepatocytes from normal rats. When cells were cultured in Dulbecco's modified Ea
Although the ability of hormones and growth factors to stimulate DNA synthesis in rat hepatocytes has been investigated extensively, no such study of human hepatocytes has been reported. Here we describe a series of experiments to identify those factors that regulate human hepatocyte DNA synthesis i