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A comparison of the high-active and low-active form of nitrate reductase in synchronousChlorella sorokiniana

✍ Scribed by Rudolf Tischner

Publisher: Springer-Verlag
Year: 1984
Tongue: English
Weight: 696 KB
Volume: 160
Category: Article
ISSN: 0032-0935
DOI: 10.1007/bf00392458

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✦ Synopsis

Chlorella sorokiniana possesses two forms of nitrate reductase (EC 1.6.6.1.). One with low activity is present in cells at the end of the light-dark cycle, the other with high activity is present after I h of illumination. The two forms can be distinguished by gel electrophoresis, isopycnic centrifugation, assay of the partial reactions and their sensitivity to antibodies, respectively. These differences are discussed with respect to an effect of intracellular nitrate on the activation of nitrate reductase.

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