A comparison of the effects of various stimulatory agents on t-PA secretion by normal and malignant cell lines

✍ Scribed by J. Clarke; B. Griffiths; J. Melling; R. Ling; T. Atkinson; A. Electricwala

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 607 KB
• Volume: 7
• Category: Article
• ISSN: 0263-6484
• DOI: 10.1002/cbf.290070407

✦ Synopsis

Succinyl con A and acetyl con A both stimulated epithelial cells to produce similar yields of tissue plasminogen activator (t-PA) to those previously obtained with native con A. However, unlike con A, the derivatized lectins did not adversely affect cell morphology and viability, and cells treated with succinyl con A could secrete t-PA for a prolonged period. Con A and the two derivatives produced similar morphological effects in Bowes melanoma cells, but t-PA production was not increased. Elevated cyclic nucleotide concentrations did not affect t-PA production from epithelial cells, but calcium ionophore treatment generated t-PA yields similar to those obtained with lectins. Azacytidine, which enhanced t-PA production from epithelial cells, did not increase yields from Bowes melanoma cells, and also sodium butyrate, reported to increase t-PA yields from human endothelial cells, had no effect on either cell line.