A comparison of the DNA fragment patterns of the mouse-virulent challenge strains and clinical isolates of Bordetella pertussis

Detergent-accelerated hydrolysis of bact

Detergent-accelerated hydrolysis of bacterial endotoxins and determination of the anomeric configuration of the glycosyl phosphate present in the “Isolated lipid A” fragment of the Bordetella pertussis endotoxin

✍ Martine Caroff; Angelika Tacken; Ladislas Szabó 📂 Article 📅 1988 🏛 Elsevier Science 🌐 English ⚖ 707 KB

Due to the formation of micelles, severance of the hydrophilic (poly-or oligosaccharide) and hydrophobic ("Lipid A") domains of bacterial lipopolysaccharides at pH 3.4 or 4.5 and 100" is slow and sometimes does not proceed at all; partially degraded fragments are usually formed. At pH 3.4 (100') in