✦ LIBER ✦

A comparison of the analytical performance of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, electrospray and matrix-assisted laser desorption/ionization mass spectrometry in the study of the protein extract from Bothrops jararaca Snake Venom

✍ Scribed by Aldo Roda; Anna Maria Gioacchini; Roberta Seraglia; Marco Montagnani; Mario Baraldini; Stefano Pedrazzini; Mario Puricelli; Pietro Traldi

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 356 KB
Volume: 11
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(199708)11:12<1297::aid-rcm963>3.0.co;2-a

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✦ Synopsis

Bothrops jararaca snake venom extract has been analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) electrospray mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) in order to obtain a rapid and accurate characterization. By the SDS-PAGE and MALDI-MS techniques about fourteen components are evidenced; with the latter technique, faster analysis and a more accurate molecular weight determination have been achieved. By ESI-MS the data obtained strongly depend on the operating conditions employed.

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