A comparison of pulsed amperometric detection and conductivity detection for carbohydrates

✍ Scribed by Lawrence E. Welch; David A. Mead Jr.; Dennis C. Johnson

• Book ID: 104100629
• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 324 KB
• Volume: 204
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/s0003-2670(00)86369-2

✦ Synopsis

Pulsed amperometric detection (PAD ) at a gold electrode and conductivity detection are compared for glucose, as a representative of the general challenge of the chromatographic detection of carbohydrates. In alkaline solutions of barium hydroxide, which are useful for anionexchange separation of carbohydrates, PAD is significantly more sensitive than the conductivity detector but the latter provides linear response to higher concentrations than those observed for PAD. The combined technique of conductivity in series with PAD is linear for glucose over the range 6 X 10F7-1 X lo-* M (ca. 6 ng-10 mg per 504 sample injection).