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A comparison of linear and branched polyethylenimine (PEI) with DCChol/DOPE liposomes for gene delivery to epithelial cells in vitro and in vivo

✍ Scribed by Wiseman, J W; Goddard, C A; McLelland, D; Colledge, W H


Book ID
110039349
Publisher
Nature Publishing Group
Year
2003
Tongue
English
Weight
203 KB
Volume
10
Category
Article
ISSN
0969-7128

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Background Efficient gene transfer is a major challenge for non-viral gene therapy. Understanding how non-viral vectors initiate gene expression could lead to the development of new future vectors with enhanced efficacy. Methods Linear or branched polyethylenimine (PEI)/DNA complexes were generated

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## Abstract Lentivirus vectors are being investigated as gene delivery vehicles for cystic fibrosis airway gene therapy. Vesicular stomatitis virus G glycoprotein (VSV‐G)‐pseudotyped vectors transduce airway epithelia via receptors that are located predominantly on the basolateral surface of the ai