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✦   LIBER   ✦

A comparison of imaging methodologies for 3D tissue engineering

✍ Scribed by Louise E. Smith; Rod Smallwood; Sheila Macneil

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
704 KB
Volume
73
Category
Article
ISSN
1059-910X

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✦ Synopsis

Abstract

Imaging of cells in two dimensions is routinely performed within cell biology and tissue engineering laboratories. When biology moves into three dimensions imaging becomes more challenging, especially when multiple cell types are used. This review compares imaging techniques used regularly in our laboratory in the culture of cells in both two and three dimensions. The techniques reviewed include phase contrast microscopy, fluorescent microscopy, confocal laser scanning microscopy, electron microscopy, and optical coherence tomography. We compare these techniques to the current β€œgold standard” for imaging three‐dimensional tissue engineered constructs, histology. Microsc. Res. Tech. 73:1123–1133, 2010. Β© 2010 Wiley‐Liss, Inc.

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