𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A Comparison of Fast-atom Bombardment and Electrospray as Methods of Ionization in the Study of Sulphated- and Sulphonated-lipids by Tandem Mass Spectrometry

✍ Scribed by W. J. Griffiths; A. Brown; R. Reimendal; Y. Yang; J. Zhang; J. Sjövall

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
653 KB
Volume
10
Category
Article
ISSN
0951-4198

No coin nor oath required. For personal study only.

✦ Synopsis

In recent years electrospray has become the most popular ionization technique for the analysis of biomolecules.

In the present study, we have compared the electrospray and continuous-flow fast-atom bombardment methods of sample introduction and ionization for the analysis of sulphated and sulphonated lipids by tandem mass spectroscopy. The tandem mass spectra obtained when using the two ionization techniques were similar; however, small differences were evident. These differences indicate that the precursor ions generated by fast-atom bombardment and electrospray have different internal energies. The two ionization techniques give similar sensitivities for the recording of collision-induced dissociation spectra. Complete structural information can be obtained from 5 to 50 ng of lipid.

📜 SIMILAR VOLUMES

Analysis of saponins from black bean by
Analysis of saponins from black bean by electrospray ionization and fast atom bombardment tandem mass spectrometry
✍ Lee, Maw-Rong; Chen, Ching-Ming; Hwang, Bao-Huey; Hsu, Li-Mei 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 137 KB 👁 2 views

Saponins from black bean (Vigna mungo L. Hepper) were analyzed using positive and negative ion fast atom bombardment mass spectrometry (FAB-MS) and liquid chromatography/mass spectrometry. Methanol was used to extract the saponins from defatted black bean, which was partially purified by extraction

Characterization of the Intermediate Pro
Characterization of the Intermediate Products of Lipid Peroxidation in Phosphatidylcholine Liposomes by Fast-atom Bombardment Mass Spectrometry and Tandem Mass Spectrometry Techniques
✍ R. Maffei Facino; M. Carini; G. Aldini; L. Colombo 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 374 KB 👁 1 views

The time-course of the HO-induced peroxidation of soybean phosphatidylcholine (PC) liposomes was monitored over 24 h by UV spectroscopy ( A 233 nm) and by fast-atom bombardment (FAB)-MS (positive-ion mode) and FAB-tandem (mass spectrometry) techniques. At the peak of maximal oxidation, a diagnostic

Direct stereochemical assignment of hexo
Direct stereochemical assignment of hexose and pentose residues in flavonoid O-glycosides by fast atom bombardment and electrospray ionization mass spectrometry
✍ Filip Cuyckens; Abdelaaty A. Shahat; Luc Pieters; Magda Claeys 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 197 KB

## Abstract Mass spectrometric methods have been developed which allow the direct stereochemical assignment of terminal monosaccharide residues in flavonoid __O__‐glycosides without the need for chemical hydrolysis. Standards containing a glucose, galactose, mannose, xylose, arabinose or apiose res