𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A comparison of chemiluminescence immunoassay and radioimmunoassay for the detection and quantification of methyltestosterone residues in meat samples

✍ Scribed by Van Peteghem, C. H. ;Van Look, L. J. ;De Guesquière, A.

Publisher
John Wiley and Sons
Year
1989
Weight
250 KB
Volume
3
Category
Article
ISSN
0884-3996

No coin nor oath required. For personal study only.

✦ Synopsis

A chemiluminescence immunoassay (CLIA) for methyltestosterone is compared with a radioimmunoassay (RIA) employing the same antiserum, raised against methyltestosterone-3-CMO-BSA and using K(4-aminobutyl)-N-ethylisoluminol conjugate of MT and i1.2-H31methyltestosterone as tracers.

Muscle tissue from slaughtered animals was selected as the matrix. After enzymatic digestion and diethylether extraction only a limited sample clean-up on Lipidex-5000 and on Bond Elut C18 was required. Both methods had similar limits of detection, sensitivities, limits of quantification and speed of analysis (working-load and availability of results).

📜 SIMILAR VOLUMES

A new enzyme immunoassay for the detecti
A new enzyme immunoassay for the detection of enteroviruses in faecal specimens
✍ Elena Terletskaia-Ladwig; Christoph Metzger; Gunnar Schalasta; Gisela Enders 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 108 KB 👁 2 views

A new enzyme immunoassay (EIA) for direct detection of enteroviruses based on a groupspecific monoclonal antibody was evaluated using stool samples from patients with suspected enteroviral infection. The EIA was compared with polymerase chain reaction (PCR) and virus isolation in cell culture. Of 20

A Survey of Sample Size Formulas for Pai
A Survey of Sample Size Formulas for Pairwise and Many-one Multiple Comparisons in the Parametric, Nonparametric and Binomial Case
✍ Manfred Horn; Rüdiger Vollandt 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 209 KB 👁 2 views

We present a survey of sample size formulas derived in other papers for pairwise comparisons of k treatments and for comparisons of k treatments with a control. We consider the calculation of sample sizes with preassigned per-pair, any-pair and all-pairs power for tests that control either the compa

Disulfide Symmetric Dimers as Stable Pre
Disulfide Symmetric Dimers as Stable Pre-Hapten Forms for Bioconjugation: A Strategy to Prepare Immunoreagents for the Detection of Sulfophenyl Carboxylate Residues in Environmental Samples
✍ M.-Carmen Estévez; Roger Galve; Francisco Sánchez-Baeza; M.-Pilar Marco 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 432 KB

## Abstract A convenient, generic synthesis of bioconjugates from haptens with a thiol group has been established. The corresponding haptens are synthesized as stable symmetric dimmers through a disulfide bond that is reduced immediately before conjugation with the aid of a di(__n__‐butyl)phenylpho