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A comparison by ultracentrifugation of the effects on DNA of ethidium bromide and of acridine orange at low ionic strength

✍ Scribed by Alfred J. Richard


Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
432 KB
Volume
27
Category
Article
ISSN
0301-4622

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The magnitude and uniformity of the torsion elastic constant (a) of linear and supercoiled pBR322 DNAs are measured in 3 mM TI+, as a function of added chloroquine/basepair ratio (chl/bp) by studying the fluorescence polarization anisotropy of intercalated ethidium dye. The time-resolved FPA is meas