A comparison between two methacrylate cements as delivery systems for bioactive human growth hormone
✍ Scribed by C. J. Goodwin; M. Braden; S. Downes; N. J. Marshall
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 733 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0957-4530
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✦ Synopsis
Two methacrylate orthopaedic bone cements were compared as delivery systems for bioactive human growth hormone (hGH). These were London Hospital Bone Cement (LHBC) and a poly(methylmethacrylate)(PMMA) bone cement. A uniquely precise bioassay for hGH, named ESTA, was used. It was adapted to assess both the bioactivity of the hormone released from hGH loaded cements and also the in vitro cytotoxicity of the cements themselves. Eluates from both cements proved cytotoxic, this being most pronounced for LHBC. The cytotoxicity could be readily diluted out, and a lengthy exposure time ( > 24 h) was required to kill the cells. Both cements released similar quantities of bioactive hGH, which were ~-0.6% of that originally incorporated. This could potentially provide significantly high local concentrations of the hormone to the tissues surrounding the implant. Approximately double quantities of hormone were released as measured by immunoassay, leading to significant decreases (p= < 0.0001)in the B:I ratios of the released hormone. The effect was greatest for LHBC. Our results suggest that the structural integrity of the hGH molecules released from LHBC were the most compromised.