A comparative study of the glutamate dehydrogenase activity in several species of marine phytoplankton
β Scribed by S. I. Ahmed; R. A. Kenner; T. T. Packard
- Publisher
- Springer-Verlag
- Year
- 1977
- Tongue
- English
- Weight
- 740 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0025-3162
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β¦ Synopsis
Kinetic studies on the NADP-linked glutamate dehydrogenase (GDH) activity in 8 species of marine phytoplankton have been carried out. The pH optima, linearity of reactions, apparent energies of activation, and apparent K m values for NADPH, NH~ and ~-ketoglutarate were determined. Based on these data, an accurate, sensitive enzyme assay procedure is presented which was successfully tested on shipboard during a recent cruise in an upwelling region. Preliminary studies on inhibition and activation of the NADP-linked GDH activity have also been carried out. In addition to NADP-linked GDH activity, NAD-linked GDH activity was discovered in certain species of phytoplankton. The possible physiological roles of NAD-and NADP-linked GDH enzymes are discussed.
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