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A comparative study of HPLC and TLC separation of amino acids using Cu(II) ion

✍ Scribed by R. Bhushan; Imran Ali; Shalini Sharma

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
266 KB
Volume
10
Category
Article
ISSN
0269-3879

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✦ Synopsis

Separation of amino acids using Cu(I1) by RP-HPLC and impregnated TLC is reported. For IIPLC the sample mixture of amino acids containing copper (11) was injected into the column while for TLC the silica gel plates were impregnated with Cu(I1). The mobile phase used in I1PLC was acetate buffer (0.3 M, pl1 (.O)-acetonitrile @:I, v h ) , and that in TLC was acetate buffer (0.3 M, pI1 6.0):acetonitrile-n-butanol (12:5:10, by vol.). The results have been compared and discussed.

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