A comparative study of edible canna (Canna edulis) starch from different cultivars. Part II. Molecular structure of amylose and amylopectin
✍ Scribed by Kittiwut Thitipraphunkul; Dudsadee Uttapap; Kuakoon Piyachomkwan; Yasuhito Takeda
- Book ID
- 104009689
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 220 KB
- Volume
- 54
- Category
- Article
- ISSN
- 0144-8617
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✦ Synopsis
Molecular structures of starches isolated from Japanese-green, Thai-green and Thai-purple cultivars of edible canna (Canna edulis Ker) were investigated. The absolute amylose content ranged from 19 to 25%. Degrees of polymerization (DPn) values of amylose determined by fluorescence-labeling method were 1590 for Thai-purple, 1620 for Japanese-green and 1650 for Thai-green cultivars. Mole% of branched fraction of amyloses from edible canna starches examined by a HPLC system after b-amylolysis of labeled amyloses was 13-16%. Branch chain-length distributions of amylopectin analyzed by HPSEC after debranching with isoamylase, followed by fluorescence-labeling of unit chain, showed bimodal distribution with the DPn range of 25 -28. The amylopectin of edible canna starches contained high amounts of organic phosphorus (391-420 ppm). The distribution profile of phosphorylated chains, separated from non-phosphorylated chains by DEAE-Sephadex A-50 chromatography, indicated that the phosphate groups were located mostly in long B-chains of amylopectin molecules.