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A compact computational model for cell construct development in perfusion culture

✍ Scribed by C.A. Chung; C.P. Chen; T.H. Lin; C.S. Tseng

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
197 KB
Volume
99
Category
Article
ISSN
0006-3592

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✦ Synopsis

Abstract

A problem nowadays tissue engineers encounter in developing sizable tissue implants is the nonuniform spread of cells and/or extracellular matrices. Research shows such a nutrients transport restriction may be improved by employing hydrodynamic culture systems. We propose a compact model for the simulation of cell growth in a porous construct under direct perfusion. Unlike the previous model proposed in the literature, which composes a cellular scaffold sandwiched between two culture media layers, the current model includes only the scaffold layer to simplify the mathematical and computational complex. Results show the present single‐layer model can predict cell spreads and the nutrient and metabolic waste distribution as accurately as does the three‐layer model. Only if the hydrodynamic aspects such as the pressure and viscous stress are prominent to know, should the more sophisticated analyses with the three‐layer model be employed. The compact model provides comparable investigations for the tissue‐engineering construct developments. Biotechnol. Bioeng. 2008;99: 1535–1541. © 2007 Wiley Periodicals, Inc.

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