A combined assay of three lysosomal marker enzymes: Acid phosphatase, β-d-glucuronidase, and β-N-acetyl-d-hexosaminidase
✍ Scribed by Bernd-Michael Löffler; Bernd Hesse; Hans Kunze
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 382 KB
- Volume
- 142
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A simplified and rapid method for simultaneous activity measurements of three lysosomal marker enzymes, acid phosphatase, beta-glucuronidase, and beta-N-acetyl-D-hexosaminidase is described. The incubation is carried out in a single test tube and stopped by adding an alkaline sodium dodecyl sulfate solution, thus avoiding centrifugations and allowing for higher Triton X-100 concentrations in the incubation media. Two products of the beta-glycosidases (phenolphthalein and 2-nitrophenolate) are measured spectrophotometrically at the respective wavelengths (555 and 420 nm), and one of the acid phosphatase products is quantitatively determined by measuring inorganic phosphate.