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A colour reaction for the differentiation of ascomycetous and hemibasidiomycetous yeasts

✍ Scribed by J. P. Walt; V. K. Hopsu-Havu

Publisher
Springer Netherlands
Year
1976
Tongue
English
Weight
393 KB
Volume
42
Category
Article
ISSN
0003-6072

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✦ Synopsis

Seventy yeast strains, representative of twenty-six ascogenous genera, four saprobic hemibasidiomycetous genera and thirteen genera of the Cryptococcales were tested for their reaction with the stabilized aromatic diazonium compound, Diazonium Blue B salt. An aqueous, buffered solution of this compound gave a characteristic red colouration with the colonies of the hemibasidiomycetous species and those Cryptococcales characterized by the hemibasidiomycetous cell-wall type. The characteristic colour reaction was not observed with colonies of either the ascomycetous yeasts or those Cryptococcales characterized by the ascomycetous cell-wall type. The possibly taxonomic use of the colour reaction with Diazonium Blue B salt as an affinitive characteristic is discussed.

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It will be seen that the high-B, white brend prepnrcd by the nid of special -Bl-rich yenst shows the lenst loss of the vitnmin during bnking, and thnt nll the brown brends, with the exception of Hovis, S ~O N a destruction of nbout one quartor to one third of the nnturally occurring -Bp I n order to