Arginase catalyzes the conversion of arginine to urea and ornithine in the liver of ureotelic animals. Higher activity of this enzyme is found in tumors as well as in the sera of patients with hepatic diseases. We have developed a simple colorimetric method for its determination. This is based on th
A colorimetric method for assaying citrulline
โ Scribed by Gerald A. Rosenthal
- Book ID
- 107711009
- Publisher
- Elsevier Science
- Year
- 1969
- Tongue
- English
- Weight
- 141 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
L-Citrulline constitutes a product of a number of enzymatic reactions. In the past a number of colorimetric methods for the determination of L-citrulline, upon its chemical modification with diacetyl monoxime at 95ยฐC, have been reported. However, all these methods are time-and material-consuming. In
Four calorimetric procedures suitable for the determination of peptidase activity on peptides having a free (Y-or e-amino group are described. Two of the methods (A and B) are modifications of the conventional ninhydrin method described by S. Moore and W. H. Stein (( 1948) J. Viol. Chem. 176, 367-38