A colony filter-hybridization procedure for the filamentous fungus Neurospora crassa

✍ Scribed by Lori L. Stohl; Alan M. Lambowitz

• Book ID: 102986919
• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 810 KB
• Volume: 134
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(83)90266-x

✦ Synopsis

A colony filter-hybridization procedure for the filamentous fungus Neurospora crassa has been developed. The procedure is sensitive enough to detect Escherichia coli plasmid pBR322 DNA integrated into chromosomal DNA in a Neurospora transformant. Thus, it should facilitate the isolation of nuclear genes by plasmid-rescue procedures.