A chromosome-specific DNA sequence which reveals a high level of RFLP in wheat

✍ Scribed by R. L. Harcourt; M. D. Gale

• Book ID: 104673176
• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 595 KB
• Volume: 81
• Category: Article
• ISSN: 0040-5752
• DOI: 10.1007/bf00228682

✦ Synopsis

An unusual genomic DNA clone, PSR454, was isolated from a partial genomic library of wheat. This sequence is moderately repeated and detects at least 30 related sequences, all located in a tight linkage block on the long arm of chromosome 3B. When used as a RFLP probe, PSR454 detects a high level of polymorphism between wheat varieties that carry the sequence. There is no detectable hybridisation to sequences in one-third of the varieties tested, providing an "on-off" polymorphism that can be detected on dot blots, rather than the more resource-consuming conventional Southern analysis.