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A chromogenic assay of substrate depletion by thiol dioxygenases

✍ Scribed by Fellner, Matthias; Doughty, Laura M.; Jameson, Guy N.L.; Wilbanks, Sigurd M.

Book ID
124109565
Publisher
Elsevier Science
Year
2014
Tongue
English
Weight
824 KB
Volume
459
Category
Article
ISSN
0003-2697

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✍ I.Yu. Filippova; E.N. Lysogorskaya; E.S. Oksenoit; G.N. Rudenskaya; V.M. Stepano πŸ“‚ Article πŸ“… 1984 πŸ› Elsevier Science 🌐 English βš– 354 KB

L-Pyroglutamyl-L-phenylalanyl-L-leucine-p-nitroanilide (PFLNA)--a convenient chromogenic substrate for assay of thiol proteinases papain, ficin, and bromelain--was prepared by enzymatic synthesis with chymotrypsin as a catalyst. The thiol proteinases hydrolyze PFLNA with the liberation of p-nitroani