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A Chitinase and a β-1,3-Glucanase Isolated from the Seeds of Cowpea (Vigna unguiculataL Walp) Inhibit the Growth of Fungi and Insect Pests of the Seed

✍ Scribed by Gomes, Valdirene M; Oliveira, Antônia Elenir A; Xavier-Filho, José


Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
527 KB
Volume
72
Category
Article
ISSN
0022-5142

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✦ Synopsis


A chitinase (22 000 Da) and a p-1,3-glucanase (26 000 Da) were isolated from cowpea (Vigna unguiculata) seeds and shown to deter development, in an in uitro assay, of the phytopathogenic fungi Colletotrichum lindemuthianum (agent of anthracnose, a disease of many plants) and Colletotrichum musae (agent of banana peel anthracnose). The isolated chitinase was also shown to negatively affect the development of the cowpea weevil (Callosobruchus maculatus) in an artificial seed system. These results suggest that the cowpea seed contains defence proteins that could be, if their levels are properly managed, utilised to promote increased protection of the plant towards attacking fungi and insects.