A Chemiluminescence Method to Analyze Phosphatidylcholine–Phospholipase Activity in Plasma Membrane Preparations and in Intact Cells

✍ Scribed by Miguel Lucas; Vı́ctor Sánchez-Margalet; Consolación Pedrera; MaLuz Bellido

• Book ID: 102560072
• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 71 KB
• Volume: 231
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1995.0051

✦ Synopsis

such as the labeling of the cell preparation or target We present a new method to analyze the hydrolysis substrate with a radioactive tracer followed by extracof phosphatidylcholine by either phospholipase C tion with organic solvents and chromatography. There-(PLC) or phospholipase D. The method is nonradioacfore, a method to assay PLC and PLD in a single step tive, rapid, and very sensitive and is based on chemilucould be quite useful in the study of signals transducminescence. It relies on the peroxidase-catalyzed chetion in cells such as neutrophils (2,3) or cellular memmiluminescent oxidation of luminol by the H 2 O 2 brane preparations.