A chemical procedure for the specific conversion of poly(adenosine diphosphate ribose) to phosphoribosyl-AMP
✍ Scribed by Peter Adamietz; Reinhard Bredehorst
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 619 KB
- Volume
- 112
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Alkaline degradation of poly(adenosine diphosphate ribose) is greatly enhanced by Mg*+ ions. Only phosphoribosyl-AMP and 5"-AMP were found as reaction products indicating exclusive and quantitative splitting of the pyrophosphate bonds. The procedure was successfully used to degrade poly(ADP-ribose) in crude cell extracts. Since complete enzymic digestion of poly(ADP-ribose) is difficult to obtain in whole tissue homogenates application of the new chemical procedure represents a significant improvement for the quantitation ofthe polymer based on the conversion to the specific derivative phosphoribosyl-AMP. It also opens a way for the determination of poly(ADP-ribose) chain length in vivo.