✦ LIBER ✦

A chemical method of labelling oligodeoxyribonucleotides with biotin: A single step procedure using a solid phase methodology

✍ Scribed by Anne M. Alves; David Holland; Michael D. Edge

Publisher: Elsevier Science
Year: 1989
Tongue: French
Weight: 234 KB
Volume: 30
Category: Article
ISSN: 0040-4039
DOI: 10.1016/s0040-4039(00)99410-3

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✦ Synopsis

Protected D(+)-biotin methylesters 3 were reduced to the corresponding alcohols 4 and phosphitylated with chloromethoxymorpholino$osphine. The resulting phosphoramidites 5 wet-5 used on a commercial DNA synthesiser to biotinylate synthetic DNA. -Chemical labelling of synthetic DNA for use as hybridisation probes continues to be of interest and a variety of methods have been reported.l,2,3,4. Typically, biotin is coupled to

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