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A Chemical Mechanism for Oscillation of Glycolytic Intermediates in Yeast Cells

✍ Scribed by Joseph Higgins


Book ID
123657605
Publisher
National Academy of Sciences
Year
1964
Tongue
English
Weight
266 KB
Volume
51
Category
Article
ISSN
0027-8424

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A method is described for rapidly quenching metabolism in yeast and extracting metabolites for analysis. The saponin digitonin is used to permeabilize the yeast cell membrane, in conjunction with perchloric acid (PCA) to quench metabolism and extract metabolites. Using this digitonin-PCA quench and