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A cell-based reporter assay for the identification of protein kinase C activators and inhibitors

✍ Scribed by Sista, Prakash (author);Edmiston, Sharon (author);Darges, James W. (author);Robinson, Simon (author);Burns, David J. (author)


Publisher
Kluwer Academic Publishers
Year
1994
Tongue
English
Weight
513 KB
Volume
141
Category
Article
ISSN
0300-8177

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A microtiter-based assay for the detecti
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A 96-well microtiter enzyme-linked immunosorbent assay (ELISA) for protein tyrosine kinases has been developed. This assay uses one of several substrates that are phosphorylated by tyrosine kinase, an antibody to phosphotyrosine, and a peroxidase-linked second antibody. Color development is monitore