𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A cell-based assay for evaluating the interaction of heparin-like molecules and basic fibroblast growth factor

✍ Scribed by Masayuki Ishihara; David J. Tyrrell; Michael C. Kiefer; Philip J. Barr; Stuart J. Swiedler


Book ID
107719009
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
641 KB
Volume
202
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


Capillary electrophoresis and enzyme sol
✍ V. Dimitrellos; F. N. Lamari; M. Militsopoulou; I. Kanakis; N. K. Karamanos πŸ“‚ Article πŸ“… 2003 πŸ› John Wiley and Sons 🌐 English βš– 97 KB

## Abstract Interaction of basic fibroblast growth factor (bFGF) with heparin/heparan sulfate proteoglycans protects the growth factor against proteolytic degradation and is essential for its cellular activity. Although the structural requirements of heparin and heparan sulfate for the high‐affinit

Interaction of heparin with human basic
✍ Andreas Sommer; Daniel B. Rifkin πŸ“‚ Article πŸ“… 1989 πŸ› John Wiley and Sons 🌐 English βš– 595 KB

Fibroblast growth factors (FGF) are a family of heparin-binding angiogenic polypeptide mitogens. In the presence of heparin, recombinant human basic fibroblast growth factor (bFGF) is fully protected from tryptic digestion and partially protected from chymotryptic digestion. Complete protection of b

Acidic and basic fibroblast growth facto
✍ Kathryn J. Brown; Ian A. Hendry; Christopher R. Parish πŸ“‚ Article πŸ“… 1995 πŸ› John Wiley and Sons 🌐 English βš– 806 KB

Heparan sulfate proteoglycans on the cell surface act as low affinity binding sites for acidic and basic fibroblast growth factor (FGF) [Moscatelli (1987): J Cell Physiol 131:123-1301 and play an important role in the interaction of FGF with the FGF receptor (FGFR). In this study, several aspects of

High and low affinity binding sites for
✍ David Moscatelli πŸ“‚ Article πŸ“… 1987 πŸ› John Wiley and Sons 🌐 English βš– 834 KB

Scatchard analysis of binding of 125I-basic fibroblast growth factor (FGF) to baby hamster kidney (BHK) cells revealed the presence of two binding sites: a high affinity site with KD of 20 pM and 80,000 sites per cell and a low affinity site with KD of about 2 nM and 600,000 sites per cell. The bind