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A cDNA clone from barley encoding the precursor from the photosystem I polypeptide PSI-G: Sequence similarity to PSI-K

✍ Scribed by Jens Sigurd Okkels; Vibeke Skovgaard Nielsen; Henrik Vibe Scheller; Birger Lindberg Møller

Book ID: 104615241
Publisher: Springer
Year: 1992
Tongue: English
Weight: 637 KB
Volume: 18
Category: Article
ISSN: 0167-4412
DOI: 10.1007/bf00019215

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✦ Synopsis

A eDNA clone encoding the photosystem I subunit, PSI-G was isolated from barley using an oligonucleotide specifying a partial amino acid sequence from a 9 kDa polypeptide of barley photosystem I. The 724 bp sequence contains an open reading frame encoding a precursor polypeptide of 15 107 kDa. Import studies using the in vitro expressed barley PsaG eDNA clone demonstrate that PSI-G migrates with an apparent molecular mass of 9 kDa on SDS-polyacrylamide gels together with PSI-C (subunit-VII). The previous assignment of the gene product of

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