A cavity-containing mutant of T4 lysozyme is stabilized by buried benzene
β Scribed by Eriksson, A. E.; Baase, W. A.; Wozniak, J. A.; Matthews, B. W.
- Book ID
- 109779461
- Publisher
- Nature Publishing Group
- Year
- 1992
- Tongue
- English
- Weight
- 357 KB
- Volume
- 355
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/355371a0
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## Abstract The structures of three mutants of bacteriophage T4 lysozyme selected using a screen designed to identify thermostable variants are described. Each of the mutants has a substitution involving threonine. Two of the variants, Thr 26 β Ser (T26S) and Thr 151 β Ser (T151S), have increased r
## Abstract An attempt has been made to identify residues in T4 phage lyoszyme that may have strained conformations and, by appropriate siteβdirected replacements, to reduce this strain and thus increase the thermostability of the protein. Valine 131, within Ξ±βhelix 126β134, was identified as a pot